| 货号: S216961 |
| 产品全名: AGR3 兔多抗 |
| 基因符号 AG3; AG-3; HAG3; hAG-3; BCMP11; PDIA18 |
| UNIPROT ID: Q8TD06 (Gene Accession – BC058284 ) |
| 背景: This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, and a C-terminal ER-retention sequence. This gene is expressed in ciliated airway epithelial cells and, in mouse, plays a role in ciliary beat frequency in multiciliated cells. This gene is also over-expressed in breast, ovarian, and prostrate cancers. [provided by RefSeq, Dec 2016] |
| 抗原: Fusion protein of human AGR3 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 216961(AGR3 Antibody) at a dilution of 1/50(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 216961(Anti-AGR3 Antibody) at dilution 1/50. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 216961(Anti-AGR3 Antibody) at a dilution of 1/50. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with fusion protein and then with D221546(Anti-AGR3 Antibody) at dilution 1/50. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: HT-29 cell lysate;
Primary antibody: 216961(AGR3 Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes | |
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