| 货号: S216390 |
| 产品全名: CA3 兔多抗 |
| 基因符号 Car3; CAIII |
| UNIPROT ID: P07451 (Gene Accession – BC004897 ) |
| 背景: Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. |
| 抗原: Fusion protein of human CA3 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 100-200;WB: 1000-5000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cell Biology, Cardiovascular |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 216390(CA3 Antibody) at a dilution of 1/120(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 216390(Anti-CA3 Antibody) at dilution 1/120. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Rat skeletal muscle tissue, Mouse skeletal muscle tissue, Human liver tissue lysates;
Primary antibody: 216390(CA3 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds | |
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