| 货号: S214616 |
| 产品全名: HIF1AN 兔多抗 |
| 基因符号 FIH1 |
| UNIPROT ID: Q9NWT6 (Gene Accession – NP_060372 ) |
| 背景: Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha. This hydroxylation event blocks the association of HIF-1alpha with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm. |
| 抗原: Synthetic peptide of human HIF1AN |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-300;WB: 500-2000;ELISA: 2000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cardiovascular, Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 214616(HIF1AN Antibody) at a dilution of 1/40(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 214616(Anti-HIF1AN Antibody) at dilution 1/40. | 
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 214616(Anti-HIF1AN Antibody) at a dilution of 1/40. | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D162109(Anti-HIF1AN Antibody) at dilution 1/40. | 
Gel: 8%SDS-PAGE, Lysate: 60 μg;
Lane 1-2: Mouse brain tissue, Mouse liver tissue;
Primary antibody: 214616(HIF1AN Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
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