| 货号: S214092 |
| 产品全名: UPF1 兔多抗 |
| 基因符号 HUPF1; NORF1; RENT1; smg-2; pNORF1 |
| UNIPROT ID: Q92900 (Gene Accession – NP_002902 ) |
| 背景: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. |
| 抗原: Synthetic peptide of human UPF1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 200-1000;ELISA: 1000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 214092(UPF1 Antibody) at a dilution of 1/35(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 214092(Anti-UPF1 Antibody) at dilution 1/35. | 
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 214092(Anti-UPF1 Antibody) at a dilution of 1/35. | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D161327(Anti-UPF1 Antibody) at dilution 1/35. | 
Gel: 6%SDS-PAGE, Lysate: 60 μg;
Lane: Raji cells;
Primary antibody: 214092(UPF1 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 minutes | |
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